development and application of aqueous two-phase partition for the recovery and separation of recombinant phenylalanine dehydrogenase

نویسندگان

hamid shahbaz mohammadi

eskander omidinia

چکیده

aqueous two-phase systems (atps) have  emerged as a powerful extraction method for the downstream processing of bio-molecules. the aim of this work was to investigate the possibility of utilizing atps for the separation of recombinant bacillus sphaericus phenylalanine dehydrogenase (phedh). polyethylene glycol (peg) and ammonium sulfate systems were selected for our experiment. the effect of different elements such as; type and concentration of peg, concentration of (nh4)2so4, ph, phase volume ratio (vr) and tie-line length (tll) on the extraction behavior and selective separation was also studied. desirable conditions for differential partitioning was obtained in 8.5 % (w/w) peg-6000, 17.5 % (w/w) (nh4)2so4 andvr 0.25at ph 8.0. phedh was mainly concentrated into the upper peg-rich phase in all tested systems. the partition coefficient (k), recovery (r %), yield (y %), tll and selectivity were found to be 58.7, 135 %, 94.42 %, 39.89 % (w/w) and 2174, respectively. from the experimental results, it was revealed that the peg molecular weight, (nh4)2so4 concentration, tll and ph of system had strong impacts on partition features. the extraction efficiency was increased with elevation of ph and tll values. in this paper, we described the partitioning behavior in peg/(nh4)2so4 atps in order to evaluate the applicability of  atps for partitioning and recovery of phedh.

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Development and Application of Aqueous Two-Phase Partition for the Recovery and Separation of Recombinant Phenylalanine Dehydrogenase

Aqueous two-phase systems (ATPS) have  emerged as a powerful extraction method for the downstream processing of bio-molecules. The aim of this work was to investigate the possibility of utilizing ATPS for the separation of recombinant Bacillus sphaericus phenylalanine dehydrogenase (PheDH). Polyethylene glycol (PEG) and ammonium sulfate systems were selected for our experi...

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rapid one-step separation and purification of recombinant phenylalanine dehydrogenase in aqueous two-phase systems

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عنوان ژورنال:
iranian journal of chemistry and chemical engineering (ijcce)

ناشر: iranian institute of research and development in chemical industries (irdci)-acecr

ISSN 1021-9986

دوره 27

شماره 2 2008

میزبانی شده توسط پلتفرم ابری doprax.com

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